ELISA Kit的准确度、精密度、灵敏度、特异性、稳定性等指标，决定了产品质量的好坏。高品质的试剂盒都需要对这些指标进行检测。目前定义高品质试剂盒一般会检测八项指标，具体信息会在本文仔细介绍。
标准一：回收率
评估试剂盒测量结果趋于真实值的程度。回收率偏高或偏低会产生假阳性或假阴性结果，影响测定值的准确性。
标准二：天然样本检测
确保试剂盒可以检测天然样本及合适的样本类型。若不对天然样本检测类型进行确定，可能会导致假阳性或假阴性结果。
标准三：交叉反应
评估试剂盒的特异性，判断其他分子与抗体的结合能力。交叉反应越大，试剂盒的特异性越差。
标准四：干扰实验
判断其他分子对抗原和抗体对相互作用的影响。干扰性越大，待测物测定值越不准确。
标准五：稳定性实验
确保试剂盒长期存放的稳定性。稳定性越好，试剂盒存储时间越长。
标准六：精密度
评估试剂盒对同一样品重复测定时能够得到形同结果的指标。精密度差的试剂盒会导致对同一样本重复检测时的结果值重现性差。
标准七：最低限检测
评估试剂盒的灵敏度，值越低试剂盒灵敏度越高。
标准八：线性实验
评估试剂盒的准确度，结果偏高或偏低，都会影响检测结果准确性。
在市场上ELISA试剂盒被分为低端、中端和高端三类，一般也是用这八项质控标准来区分的。仅有线性实验检测报告的试剂盒属于低端产品，完成精密度、最低检测限、回收率检测的试剂盒属于中端产品，完成交叉实验、干扰实验、稳定性、天然样本检测等八项指标检测的试剂盒属于高端产品。
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