经常在实验室跑PCR的同学看过来了,今天我们汇总一下那些设计引物的软件或者网站,如果你们还有其他好用的工具,欢迎补充~
1. primer premier
一款专业的引物设计软件,具有PCR或测序引物以及杂交探针设计功能。可以简单地通过手动拖动鼠标以扩增出相应片段所需的引物,而在手动的任何时候,下面显示各种参数的改变和可能的二聚体、异 二聚体、发夹结构等。也可以给定条件,让软件自动搜索引物,并将引物分析结果显示出来。
2. FastPCRy
一款快速设计各种类型PCR引物的工具,包括一些常用的DNA与蛋白序列软件工具。基于在标准和长PCR、反向PCR引物的设计新方法,直接氨基酸序列的简并PCR、多重PCR和电子PCR;序列比对、聚类和任何重复序列搜索。
3. Beacon Desiner
Beacon Designer是一款功能强大且简单易用的pcr引物设计软件,它可以通过多次的验证,为你节约很多验证的时候,从而节约经费,是Real-time PCR的必备武器。可用于SYBRGreen试验设计、HRMA试验设计、双标签探针设计。
4. Oligo软件
Oligo软件主要特点就是傻瓜、简单、好用。下载途径与使用方法见下方地址:
http://www.omicshare.com/forum/thread-846-1-7.html。
5. BatchPrimer
一个批量设计引物的在线工具,一次可以设计500条序列引物,并且是在线的,完全免费,速度也是杠杠的。具体教程戳这里查看,但文章介绍的版本可能旧了,建议大家download最新版来用。
6. 植物方面的引物设计方法
这就是我们去年转载的一个帖子,没看过的同学可以戳原标题查看:不会设计引物肿么破?点这里呀,已看过的同学忽略……
7. 用NCBI设计引物
这也是已经分享过的方法,戳原标题可查看:如何快速从转录组结果中挑基因设计引物,你也可以跳过~
8. GenScript Primer Design
网址:https://www.genscript.com/ssl-bin/app/primer
这个在线工具可以设计引物和探针实时荧光定量PCR(TaqMan),可自定义潜在的PCR扩增产物的大小范围,Tm(熔融温度)的引物和探针,以及生物体。
左侧一栏单击 Real-Time PCR Primer Design — PCR 扩增size 一般设置为100-150bp — primeTM值一般都设置成60℃– 把CDS序列复制到下面的方框中 — start即可。默认前面两个引物最好。
9. Primer Quest
网址:http://sg.idtdna.com/Primerquest/Home/Index
最后安利一个简单好用的在线网站,界面比较简单,可输入序列或者上传excel表格,不再详细介绍了。
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