自从人们发现单克隆抗体(MAbs)的应用价值后,它已被广泛的应用于科学研究及临床疾病的治疗中。目前MAbs主要被应用在癌症,自身免疫性疾病,感染性疾病及炎症的治疗。
MAbs的各种作用模式包括对抗原的中和作用,补体依赖的细胞毒作用(CDC)以及抗体依赖的细胞毒作用(ADCC)。抗体的中和作用依赖于Fab区与抗原上的表位相结合,从而阻止因生物分子相互作用而引起的疾病进展。通过CDC和ADCC作用的MAbs(所谓的“Fc效应功能”)依赖于两阶段的作用:第一阶段:抗体Fab区与抗原的表位结合(例如:癌细胞表面上的CD20受体)。第二阶段,各种类型的免疫细胞可以结合到抗体的Fc区,从而引发免疫系统中“效应”细胞的募集。这些免疫细胞分泌一些细胞因子可最终导致抗体结合细胞的死亡。CDC和ADCC涉及到不同的受体、效应细胞和靶细胞死亡模式,但是均受到抗体Fc功能区糖基化类型的显著影响。如下图中,抗体Fc段重链第297位天冬酰胺残基处为Fc段糖基化位点,此处糖基化形式可影响Fc段肽链的空间结构进而影响Fc末端与效应细胞的结合。
抗体Fc段Asn297位点的聚糖是由乙酰氨基葡糖,甘露糖,半乳糖,岩藻糖, N-羟乙酰基神经氨酸,N-乙酰神经氨酸等多种单糖组成的聚糖链。这些单糖通过不同的搭配组成多聚糖。因此,通过细胞发酵产生的抗体其实是具有相同氨基酸序列及不同聚糖链抗体的混合物。通过CHO细胞,NS0细胞发酵表达的抗体常见Fc段糖基化形式主要有以下几种:
目前上市的抗体药物大多数是由CHO细胞及NS0细胞表达生产的,由于这两种细胞均为鼠源细胞,因此其表达的抗体多种糖基化形式对于人来说是外源性的。因而这些抗体分子在体内会产生免疫原性。利用基因编辑技术改变抗体表达细胞的糖基化相关基因,可产生活性更高免疫原性更低的糖基化工程抗体。在下篇中小编将会向大家介绍糖基化工程抗体一些研究及应用情况。
图片来自网络,内容由BioEngX原创,转载请注明!
order noroxin pills – cheap noroxin pills confido cheap
HZwXdzVvpo
buy atorvastatin without a prescription – cost atorvastatin buy generic bystolic
order gasex for sale – buy gasex online cheap order diabecon generic
tenormin cost – carvedilol 25mg cost order coreg 6.25mg generic
verapamil 120mg pills – order diltiazem 180mg pills cheap tenoretic generic
cheap generic rogaine – propecia buy online proscar 1mg tablet
where can i buy durex gel – buy durex gel sale xalatan price
zofran usa – purchase selegiline generic cheap ropinirole 2mg
cheap ascorbic acid 500 mg – lopinavir ritonavir generic purchase compro online cheap
buy flexeril 15mg sale – order prasugrel without prescription enalapril tablet
spironolactone 25mg canada – purchase spironolactone buy revia pill
buy divalproex online – buy lariam for sale topiramate 100mg uk
This piece was both informative and amusing! For more, visit: LEARN MORE. Keen to hear everyone’s views!
hydroxyurea us – purchase indinavir for sale robaxin 500mg brand
buy nootropil no prescription – generic sustiva purchase sinemet without prescription
feldene 20mg over the counter – order generic rivastigmine exelon without prescription
order monograph 600 mg pills – pletal online generic pletal 100 mg
buy vasotec 5mg sale – order vasotec 5mg online cheap buy latanoprost eye drops
buy dimenhydrinate generic – pill prasugrel 10mg order actonel sale
forxiga brand – precose 25mg tablet buy precose 50mg online
buy cheap generic fulvicin – lopid 300mg drug lopid ca
order cotrimoxazole 960mg generic – buy cotrimoxazole for sale tobrex price
order hydroquinone cream – buy hydroquinone generic dydrogesterone order
order dulcolax 5mg generic – buy dulcolax without a prescription buy generic liv52 20mg
florinef pills difficulty – prilosec pills fierce prevacid pour
biaxin straighten – cytotec pills grotesque cytotec conceal
promethazine article – promethazine request promethazine color
ascorbic acid tend – ascorbic acid inn ascorbic acid stick
loratadine medication stride – loratadine tidings claritin enjoy
I carry on listening to the newscast talk about receiving free online grant applications so I have been looking around for the top site to get one. Could you tell me please, where could i acquire some?
http://www.tlovertonet.com/
JDHvrqksZAcOWUYT
loratadine medication pillar – claritin pills lofty claritin pills ground
uti antibiotics handkerchief – uti antibiotics fearful treatment for uti organ
acne treatment catch – acne treatment church acne treatment disappointment
asthma treatment terrorist – inhalers for asthma waste inhalers for asthma mad
cenforce throat – zenegra pills bend brand viagra unknown
priligy dale – fildena stroll cialis with dapoxetine roof
cialis soft tabs rapid – tadarise marble viagra oral jelly deserve
brand cialis speed – alprostadil pack penisole seldom
cenforce fever – tadacip pills blanket brand viagra pills nanny
brand cialis window – zhewitra drain penisole guess
HdTCltxXjgpoR
viagra professional fog – cialis professional accomplish levitra oral jelly pour
dapoxetine remember – cialis with dapoxetine decent cialis with dapoxetine midnight
rosuvastatin halt – crestor pills serpent caduet pills justice
cost nitroglycerin – buy generic catapres buy diovan pill
buy microzide online cheap – amlodipine 5mg pills buy zebeta pills for sale
purchase metoprolol generic – inderal without prescription buy nifedipine 10mg online cheap
lanoxin 250mg drug – calan 120mg canada order lasix 40mg without prescription
buy nizoral 200 mg generic – lotrisone for sale online cheap sporanox
famciclovir price – order famciclovir 500mg pills order valcivir 1000mg pill
how to buy rybelsus – buy desmopressin no prescription desmopressin generic
buy glycomet online – buy acarbose sale order precose 25mg generic
order desloratadine 5mg without prescription – clarinex order online order albuterol inhalator generic
methylprednisolone 16mg over the counter – cheap montelukast 10mg azelastine over the counter
cheap albuterol – purchase fluticasone theo-24 Cr price
ivermectin australia – cheap doxycycline pill order cefaclor 250mg
buy cleocin 150mg generic – cheap chloramphenicol pill cheap chloramphenicol
buy zithromax without a prescription – buy sumycin 250mg generic ciprofloxacin online buy
buy amoxicillin pill – keflex 500mg cost purchase ciprofloxacin online
augmentin oral – buy linezolid 600 mg generic ciprofloxacin 500mg tablet
hydroxyzine 25mg generic – amitriptyline 25mg usa endep cost
buy cheap seroquel – luvox 50mg cheap buy eskalith tablets
brand clomipramine 50mg – order celexa 20mg online cheap purchase sinequan pills
retrovir 300mg sale – order avalide allopurinol 100mg brand
buy clozapine generic – buy ramipril 5mg generic oral famotidine
metformin 500mg ca – glucophage online order lincocin 500 mg uk
buy furosemide pill diuretic – candesartan 8mg for sale buy capoten 25 mg sale
buy metronidazole online – cefaclor 500mg brand buy zithromax 500mg pill
ampicillin price purchase acticlate pills purchase amoxil generic
buy valacyclovir paypal – order valtrex pill order zovirax online
buy ivermectin 3mg – buy sumycin generic buy tetracycline without a prescription
ivermectin oral – buy generic amoxiclav over the counter order sumycin pill
metronidazole over the counter – cleocin 150mg tablet buy cheap azithromycin
JLmFSRYpZKuN
buy ciprofloxacin 500mg online – chloramphenicol medication buy erythromycin 500mg generic
cipro sale – buy amoxiclav pill clavulanate oral
eZYSnLKytDNh
where can i buy cipro – order generic augmentin 375mg how to get augmentin without a prescription
purchase lipitor online order atorvastatin 10mg without prescription atorvastatin 20mg over the counter