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A:请教一下各位大神,为什么这个P450在大肠杆菌表达,N端的Met会被切掉?谢谢
B:大肠内部的酶系统切除的
C:跟第二个氨基酸的种类有很大关系
D:应该是大肠杆菌的N end rule,第一个M很多情况都被切掉。
E:是被甲硫氨酸氨基肽酶切除的。切除与否有其具体的规律: 一、如果 N-端的第二位氨基酸残基的回转半径≤1.29Å,如甘氨酸、丙氨酸、 丝氨酸、半胱氨酸、苏氨酸、脯氨酸和缬氨酸,那么起始的甲硫氨酸将会被切除; 但如果临近的 N-末端第二位氨基酸残基的回转半径≥1.43 Å 时,甲硫氨酸则不会 被切除。 二、如果 N-末端的第三位氨基酸残基是脯氨酸,那么甲硫氨酸从具有中等 长度侧链的氨基酸残基上切割下来则会受到抑制,而如果 N-末端序列是 Met-Cys-Pro-和 Met-Pro-Pro-,起始的甲硫氨酸则不会被切割下来,这或许是因 为第三位的脯氨酸残基会在拓扑结构上扭曲氨基末端,因此会抑制甲硫氨酸氨基肽酶的作用。
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