糖与肽键合的方式及位点具多样化。首先从糖组分看，既可以由异头碳上的氧原子也可由该碳原子上的氮与肽键合，既可以由单糖形式也可以由双糖及寡聚糖形式与肽键合。从肽的底物结构看，含羟基侧链的残基、含氨基侧链的残基、含羧基侧链甚至含酰胺侧链的残基均可以各自的方式与糖键合(图1)
图1 糖与含羧基肽及含氨基肽的键合
例1 把D-葡萄糖衍生成含噁唑啉结构的中间体，再与固相载体上含Ser、Thr或Tyr残基的肽键合（图2）。
图2糖与含Ser残基肽链的固相键合
例2 肽链上含有多个含侧链一COOH残基而又只对其中的一个(或几个)进行糖化时，可用正交方式保护不同的一COOH。在下列的壳二糖糖肽合成中，对被糖化的ASp给以烯丙酯保护方式(可用Pd脱除)。而旁边的Glu则用叔丁酯形式保护。后者对pd很稳定，不干扰AsP的糖化反应（图3）。
图3含多个侧链一COOH的选择性糖化合成
例3 除了前面的先组装肽链后糖化方式外，还可以利用硅醚Linker先使糖组分与载体相连。随后组装成二糖，后者经活化后再与肽段上的Asp侧链一COOH相键合（图4）。
图4硅醚Linker糖组分与肤的键合
例4 以三烷基硅氯为Linker前体，在碘化钠催化下与保护氨基酸的侧链羟基或糖的羟基形成硅醚键。此Linker结构与固相载体相连后，可以在C端或N端两个不同方向上进行肽链或糖肽的组装合成。基本方式如下所示（图5）。
图5
杭州专肽生物官方微信：allpeptide2
generic lamisil 250mg – order diflucan online cheap griseofulvin medication
buy generic semaglutide – order DDAVP generic DDAVP price
purchase micronase without prescription – order glucotrol 5mg generic dapagliflozin 10mg pills
desloratadine over the counter – buy ketotifen generic buy albuterol no prescription
medrol 4 mg over counter – azelastine for sale buy azelastine 10ml sale
purchase albuterol online – allegra generic theophylline price
buy ivermectin 6mg for humans – buy cefaclor online cheap cefaclor order online
cleocin 300mg price – cheap cleocin purchase chloromycetin sale
zithromax 250mg canada – flagyl where to buy ciplox 500mg us
buy amoxicillin for sale – buy cheap generic trimox cipro 500mg cost
augmentin 375mg cost – linezolid 600mg cheap order cipro 500mg sale
hydroxyzine 10mg uk – cheap buspar 10mg buy endep 25mg online cheap
seroquel without prescription – bupropion oral eskalith pills
buy anafranil 50mg online cheap – order cymbalta 40mg pills sinequan 25mg cost
generic retrovir 300mg – order allopurinol 300mg for sale oral zyloprim 300mg
purchase clozapine sale – oral quinapril 10mg famotidine tablet
order glycomet online cheap – order glycomet 500mg online lincomycin 500 mg uk
buy lasix paypal – how to buy coumadin cheap captopril 25 mg
buy flagyl 400mg without prescription – buy zithromax 500mg generic buy azithromycin 500mg online
buy acillin pill buy acillin for sale how to get amoxicillin without a prescription
valacyclovir 500mg pills – diltiazem brand acyclovir 800mg oral
buy ivermectin for humans – buy sumycin 250mg generic purchase sumycin sale
ivermectin 12mg tablets – generic sumycin order tetracycline 500mg pill
metronidazole order online – amoxicillin drug buy azithromycin online
purchase ciplox – purchase amoxicillin generic
buy erythromycin 250mg without prescription
ciprofloxacin 1000mg cheap – bactrim 960mg us augmentin order online
order cipro 1000mg pills – purchase cipro generic amoxiclav us
order lipitor 40mg sale purchase atorvastatin for sale purchase atorvastatin generic