蛋白质研究的小伙伴们肯定经常接触到一个名词,等电点(isoelectric point)。当蛋白质处于某一pH环境中,所带正、负电荷恰好相等,即净电荷为零,呈兼性离子,此时溶液的pH值被称为蛋白质的等电点。
蛋白质在等电点时,其溶解度最小,最易形成沉淀物,主要应用于蛋白质等两性电解质的分离提纯。
让不少小伙伴抓破头的问题是,如何计算一个蛋白质的理论等电点呢?下面就由小艾为您推荐等电点计算工具:https://web.expasy.org/protparam/
如上图,您可以有2种方式来查阅一个蛋白质的等电点:
方法一
在上图①的文本框中,输入Swiss-Prot /TrEMBL数据库的蛋白ID, 例如:P05130——点击计算(Compute Parameters) ——-您可以分析全长蛋白,或者部分结构域的序列的等电点。点击下图的序列范围。
方法二
在上图②的文本框中,输入蛋白质的纯序列(氨基酸的一个字母的代码),可参考Uniprot数据库的fasta序列例如:MSEGSDNNGDPQQQKKKNVFN… ——–点击计算(Compute Parameters) —–直接得出结果。
好了,小伙伴们不用再往下翻了,分分钟就把理论等电点计算出来啦~ 全文结束!
更多资讯,欢迎持续关注每日生物评论(Bio-review)哦~
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