话不多说，直接上实验室里常见的几种缓冲液的配比方法
1，Trizma base 和Trizma HCl (Sigma 的Tris配比方法) 混合配1 L 50 mM Tris 缓冲液
2，1L 1M磷酸钠缓冲液（pH6.0-7.2）配比表（25°C）
1 L 100 mM 磷酸钠缓冲液（pH5.8-8.0）配比表（25°C）
3，50 mM Glycine-HCl 缓冲液
准备200 mM glycine溶液（A），200 mM HCl （B）
50 mL (A) + X mL (B)，稀释到200 mL
pH范围表如下所示
4，50 mM Glycine-NaOH缓冲液
准备200 mM glycine溶液 (A)，200 mM NaOH (B)
50 mL (A) + X mL (B)，稀释到200 mL
pH范围表如下所示
5，50 mM 乙酸根缓冲液
准备200 mM 乙酸溶液 (A)，200 mM乙酸钠溶液 (B)
X mL (A) + Y mL (B), 稀释到100 mL
pH范围如下所示
6，50 mM 柠檬酸盐缓冲液
准备100 mM柠檬酸溶液(A)，100 mM柠檬酸钠溶液(B)
X mL (A) + Y mL (B)，稀释到100 mL
pH范围如下表所示
7，50 mM 碳酸根缓冲液
准备200 mM Na2CO3 (A) 和200 mM NaHCO3 (B)
X mL (A) + Y mL (B), 稀释到200 mL
pH范围表如下所示
8，PBS
然后调节pH到需求的值，最后使用蒸锅或过滤膜进行杀菌。
要说缓冲液种类，肯定不是只有这八种，小编只是在这里简单举例了这八种，最后附上一张各种缓冲液适用的pH范围，快来看看你用的缓冲液在不在这里面吧~~
最后小编诚信推荐一个关于缓冲液配比和配比方法的权威网站，冷泉港分子生物学试剂制备大全：http://cshprotocols.cshlp.org/site/recipes/
留着各位慢慢享用吧！
管理员邮箱：info@bioengx.org；管理员微信：bioengxadmin; 内容由作者原创，部分图片来自网络。欢迎留言讨论哦，如需再转载，请联系管理员。
扫描下方二维码关注BioEngX官方微信公众平台
buy nizoral pills – lotrisone online buy brand sporanox
rybelsus usa – desmopressin order DDAVP buy online
buy generic lamisil – fulvicin 250mg uk griseofulvin canada
buy repaglinide 2mg sale – buy generic repaglinide buy empagliflozin without prescription
order micronase generic – buy forxiga paypal buy forxiga 10 mg generic
buy methylprednisolone 8 mg online – order montelukast sale order azelastine 10 ml
buy clarinex sale – buy clarinex 5mg pill order ventolin 2mg for sale
albuterol for sale online – buy promethazine medication buy theo-24 Cr 400 mg without prescription
ivermectine online – order stromectol online order cefaclor 250mg without prescription
azithromycin 500mg sale – order tetracycline without prescription buy generic ciprofloxacin
buy cleocin 150mg generic – chloromycetin online order buy generic chloromycetin
buy amoxicillin generic – buy cheap duricef ciprofloxacin 1000mg cost
augmentin brand – buy ampicillin generic cost cipro
cost atarax 25mg – sarafem 40mg pill buy endep 10mg generic
order clomipramine 50mg pills – order amoxapine 50 mg pills purchase doxepin
seroquel drug – purchase luvox pills eskalith online
clozapine ca – purchase frumil pills pepcid 40mg price
zidovudine cost – glucophage 1000mg over the counter buy generic allopurinol online
glycomet 1000mg pills – generic glycomet 500mg buy lincomycin medication
buy lasix tablets – order capoten 25mg generic cheap capoten
brand acillin purchase penicillin sale amoxil uk
ivermectin pills for humans – order ciprofloxacin oral sumycin 500mg
valtrex cheap – buy zovirax no prescription order zovirax 800mg pill
buy generic ciplox – doxycycline drug order erythromycin 250mg without prescription
flagyl brand – buy cleocin 300mg online cheap where to buy azithromycin without a prescription
order ciprofloxacin pill – order ethambutol 1000mg generic augmentin
cipro usa – oral cephalexin 500mg clavulanate pills
buy atorvastatin online cheap atorvastatin pills order atorvastatin 20mg generic
醋酸根的那个是不是错了呀，终浓度应该是100mM吧？