话不多说,直接上实验室里常见的几种缓冲液的配比方法
1,Trizma base 和Trizma HCl (Sigma 的Tris配比方法) 混合配1 L 50 mM Tris 缓冲液
2,1L 1M磷酸钠缓冲液(pH6.0-7.2)配比表(25°C)
1 L 100 mM 磷酸钠缓冲液(pH5.8-8.0)配比表(25°C)
3,50 mM Glycine-HCl 缓冲液
准备200 mM glycine溶液(A),200 mM HCl (B)
50 mL (A) + X mL (B),稀释到200 mL
pH范围表如下所示
4,50 mM Glycine-NaOH缓冲液
准备200 mM glycine溶液 (A),200 mM NaOH (B)
50 mL (A) + X mL (B),稀释到200 mL
pH范围表如下所示
5,50 mM 乙酸根缓冲液
准备200 mM 乙酸溶液 (A),200 mM乙酸钠溶液 (B)
X mL (A) + Y mL (B), 稀释到100 mL
pH范围如下所示
6,50 mM 柠檬酸盐缓冲液
准备100 mM柠檬酸溶液(A),100 mM柠檬酸钠溶液(B)
X mL (A) + Y mL (B),稀释到100 mL
pH范围如下表所示
7,50 mM 碳酸根缓冲液
准备200 mM Na2CO3 (A) 和200 mM NaHCO3 (B)
X mL (A) + Y mL (B), 稀释到200 mL
pH范围表如下所示
8,PBS
然后调节pH到需求的值,最后使用蒸锅或过滤膜进行杀菌。
要说缓冲液种类,肯定不是只有这八种,小编只是在这里简单举例了这八种,最后附上一张各种缓冲液适用的pH范围,快来看看你用的缓冲液在不在这里面吧~~
最后小编诚信推荐一个关于缓冲液配比和配比方法的权威网站,冷泉港分子生物学试剂制备大全:http://cshprotocols.cshlp.org/site/recipes/
留着各位慢慢享用吧!
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醋酸根的那个是不是错了呀,终浓度应该是100mM吧?