肌醇作为一种高附加值产品,已经被广泛应用于医药、饲料、食品以及化妆品等行业。酶法高效生产高附加值产品是先进生物制造的重要手段,而多酶级联反应更是被誉为“下一代的生物催化技术”。中国科学院微生物研究所陶勇研究组构建了一个包含多聚磷酸葡萄糖激酶、1-磷酸肌醇合成酶和肌醇单磷酸化酶的三酶级联反应体系,实现了由葡萄糖和多聚磷酸合成高附加值产品——肌醇。
利用该三酶级联反应体系,由葡萄糖和多聚磷酸合成肌醇不需要ATP和辅因子。由多聚磷酸提供磷酸基团,并在多聚磷酸葡萄糖激酶(PPGK)的作用下实现葡萄糖的磷酸化,然后经过关键酶1-磷酸肌醇合成酶(IPS)的环化和异构化作用,6-磷酸葡萄糖(G-6P)转化为3-磷酸肌醇(I-3P),最后在肌醇单磷酸化酶(IMP)的去磷酸化作用下实现肌醇的合成。
该研究实现了肌醇的三酶级联合成,为进一步构建“细胞工厂”,实现肌醇的细胞法合成打下了基础。同时也为肌醇的下游产物(如鲨肌醇、葡萄糖醛酸等)的生物合成指明了方向。
该研究成果发表在Enzyme and Microbial Technology期刊,并得到了中科院重点部署项目(No.ZDRW-ZS-2016-3-1)的资助。中国科学院微生物生理与代谢工程重点实验室陶勇研究组主要从事工业微生物代谢工程的基础和应用研究工作,通过代谢工程、合成生物学等手段对大肠杆菌、酵母等微生物进行改造,构建高效的全细胞催化剂生产高附加值化学品。
文章链接:https://www.sciencedirect.com/science/article/pii/S0141022918300127
本文作者信息:王雷,电话18322693935,单位:中国科学院微微生物研究所
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