2017年4月11日,天津市千人计划专家张以恒实验室在biotechnology and bioengineering杂志发表了体外合成生物学最新文章,An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch。该文报道了第一个在工业规模,通过体外合成生物学平台生产的生物分子,肌醇(inositol)。
肌醇(维生素B8)主要应用于医药、食品、化妆品、饲料等工业领域。传统生产肌醇的方法主要是通过酸法水解肌醇六磷酸(phytate)进行。这种方法存在很多的缺陷,比如原料费用高昂,严重的磷酸污染,产物分离困难等。
本文报道了一个体外的多酶分子通路,从淀粉直接生成肌醇。如下图所示:
这个通路一共包括了四个来源于超嗜热菌株的酶分子,分别是alpha-葡聚糖磷酸化酶(alpha-glucan phosphorylase),葡萄糖磷酸变位酶(phosphoglucomutase),肌醇-1-磷酸合成酶(inositol 1-phosphate synthase)以及肌醇单磷酸酶(inositol monophosphatase)。这四种酶在大肠杆菌BL21(DE3)中实现了高效的表达,同时能够很容易地通过热处理实现蛋白的纯化。
这一多酶通路在20000L发酵罐中成功地实现了淀粉到肌醇的转化,48小时反应后,获得了95g/L的肌醇产量。该生产方法大大降低了肌醇生产的成本,节省了传统的基于肌醇六磷酸酸解法约三分之二的操作费用。同时,相比于传统的方法,采用体外合成生物学操作平台生产肌醇方法可以降低约500倍的磷酸污染。
这一研究证实了利用体外合成生物学平台进行工业化生产地有效性,以及利用高效平台生产高附加价值产品的前景。
viagra professional online quite – buy viagra professional infinite levitra oral jelly kitty
crestor traffic – ezetimibe online return caduet pills content
simvastatin snow – order gemfibrozil 300 mg online cheap lipitor fry
brand hydrochlorothiazide – buy lisinopril 10mg pills zebeta pills
buy generic lopressor 50mg – nifedipine order online buy adalat generic
buy lanoxin sale – order verapamil generic oral lasix 40mg
famvir cost – order generic valcivir 1000mg valcivir sale
order nizoral online – nizoral 200 mg canada sporanox 100 mg pills
terbinafine pills – fulvicin canada grifulvin v tablet
purchase semaglutide pill – where to buy desmopressin without a prescription buy cheap generic desmopressin
prandin 2mg cost – pill empagliflozin empagliflozin 10mg uk
order micronase for sale – order pioglitazone pills order forxiga online
order generic clarinex – purchase ventolin inhalator for sale albuterol 2mg cost
depo-medrol cost – how to get astelin without a prescription buy generic azelastine
albuterol drug – order seroflo without prescription theo-24 Cr 400mg ca
cleocin online order – order generic vantin purchase chloromycetin without prescription
buy generic zithromax for sale – azithromycin 250mg cost buy ciprofloxacin paypal
buy amoxil generic – order generic duricef 250mg buy ciprofloxacin 500mg online cheap
augmentin canada – augmentin price baycip pills
buy hydroxyzine 25mg online cheap – order pamelor pill buy endep tablets
anafranil over the counter – buy anafranil 25mg pill doxepin 25mg price
buy quetiapine 50mg – cheap bupron SR generic eskalith without prescription
how to buy clozaril – order famotidine 40mg pill pepcid canada
order zidovudine 300 mg online pill – generic irbesartan buy allopurinol 100mg sale
glycomet 1000mg cost – sulfamethoxazole uk order lincocin 500 mg online cheap
lasix uk – order furosemide 40mg generic capoten 25 mg brand
metronidazole over the counter – buy clindamycin no prescription order zithromax 500mg pills
ampicillin online penicillin oral order amoxil pills
order valtrex 1000mg pill – how to buy diltiazem order zovirax without prescription
stromectol generic name – sumycin over the counter buy generic tetracycline 250mg
flagyl drug – buy generic zithromax cost azithromycin
generic ciplox – cheap chloramphenicol pill buy generic erythromycin 500mg
ciprofloxacin 500mg pills – order septra pills buy clavulanate cheap
ciprofloxacin 500mg brand – amoxiclav ca augmentin 375mg canada
lipitor drug atorvastatin without prescription oral lipitor